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Astatke M

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Publications:

Title Authors Year Journal
Mutant Thermotoga neapolitana DNA polymerase I: altered catalytic properties for non-templated nucleotide addition and incorporation of correct nucleotides. Astatke M 2002 Nucleic acids research
Side chains that influence fidelity at the polymerase active site of Escherichia coli DNA polymerase I (Klenow fragment). Astatke M 1999 The Journal of biological chemistry
How E. coli DNA polymerase I (Klenow fragment) distinguishes between deoxy- and dideoxynucleotides. Astatke M 1998 Journal of molecular biology
A single side chain prevents Escherichia coli DNA polymerase I (Klenow fragment) from incorporating ribonucleotides. Astatke M 1998 Proceedings of the National Academy of Sciences of the United States of America
A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. Astatke M 1996 The Journal of biological chemistry
Deoxynucleoside triphosphate and pyrophosphate binding sites in the catalytically competent ternary complex for the polymerase reaction catalyzed by DNA polymerase I (Klenow fragment). Astatke M 1995 The Journal of biological chemistry
Re-engineering the polymerase domain of Klenow fragment and evaluation of overproduction and purification strategies. Astatke M 1993 Nucleic acids research

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