Phi29
From
Bacteriophage φ29
(aliases:φ29)
Welcome to the Polymerase Page
This page presents all the information in Polbase for Phi29.
Mutants:At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.
Structures/Sequence:If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.
External links:Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.
References:All references that refer to this Polymerase are listed with their topics and paper's title in the References table.
Results:All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.
Help icons:
The symbol in the upper-right corner of the page
links to this help text. The question mark icon is used everywhere to indicate that help is available.
Phi29
is a family B enzyme
from
Bacteriophage φ29.
Selected Properties for Phi29: |
3'-5' exo activity | 5'-3' exo activity | Frameshift Rate | Substitution Rate | General Error Rate |
|---|---|---|---|---|---|
| Yes (8) | no data | no data | no data | 3.0e-06 errors/bp (1) |
- References (146)
- Structures (8)
- Sequences (1)
- Mutants (19)...
- Formulations (0)
- Polymerase Lots (0)
- External links
| Year | Authors | Title | Results by property |
|---|---|---|---|
| 2013 | Alicia Del Prado, José María Lázaro, Laurentino Villar, Margarita Salas, Miguel de Vega | Dual Role of φ29 DNA Polymerase Lys52... | |
| 2012 | Elizabeth A Manrao, Ian M Derrington, Andrew H Laszlo, Kyle W Langford, Matthew K Hopper, Nathaniel Gillgren, Mikhail Pavlenok, Michael Niederweis, Jens H Gundlach | Reading DNA at single-nucleotide reso... | |
| 2012 | José A Morin, Francisco J Cao, José María Lázaro, J Ricardo Arias-Gonzalez, José M Valpuesta, José L Carrascosa, Margarita Salas, Borja Ibarra | Active DNA unwinding dynamics during ... | |
| 2012 | Joseph M Dahl, Ai H Mai, Gerald M Cherf, Nahid N Jetha, Daniel R Garalde, Andre Marziali, Mark Akeson, Hongyun Wang, Kate R Lieberman | Direct observation of translocation i... | |
| 2012 | Kate R Lieberman, Joseph M Dahl, Ai H Mai, Mark Akeson, Hongyun Wang | Dynamics of the translocation step me... | |
| 2012 | Xu Hun, Fang Liu, Zhenhua Mei, Lifeng Ma, Zhouping Wang, Xiliang Luo | Signal amplified strategy based on ta... | |
| 2011 | Mario Mencía, Pablo Gella, Ana Camacho, Miguel de Vega, Margarita Salas | Terminal protein-primed amplification... | |
| 2011 | Noël P Burtt | Whole-genome amplification using Φ29 ... | |
| 2010 | Patricia Pérez-Arnaiz, José María Lázaro, Margarita Salas, Miguel de Vega | phi29 DNA polymerase active site: rol... | |
| 2010 | Miguel de Vega, José María Lázaro, Mario Mencía, Luis Blanco, Margarita Salas | Improvement of phi29 DNA polymerase a... | |
| 2010 | Kate R Lieberman, Gerald M Cherf, Michael J Doody, Felix Olasagasti, Yvette Kolodji, Mark Akeson | Processive replication of single DNA ... | |
| 2009 | Arunas Lagunavicius, Egle Merkiene, Zivile Kiveryte, Agne Savaneviciute, Vilma Zimbaite-Ruskuliene, Tomas Radzvilavicius, Arvydas Janulaitis | Novel application of Phi29 DNA polyme... | |
| 2009 | Irene Rodríguez, José María Lázaro, Margarita Salas, Miguel de Vega | Involvement of the TPR2 subdomain mov... | |
| 2009 | Patricia Pérez-Arnaiz, José María Lázaro, Margarita Salas, Miguel de Vega | Functional importance of bacteriophag... | 3-5' exo,5-3' exo,All properties |
| 2008 | Margarita Salas, Luis Blanco, José María Lázaro, Miguel de Vega | The bacteriophage phi29 DNA polymerase. | |
| 2008 | Yun Xu, Simon Gao, John F Bruno, Benjamin J Luft, John J Dunn | Rapid detection and identification of... | |
| 2008 | Kaisa Silander, Janna Saarela | Whole genome amplification with Phi29... | |
| 2008 | Arunas Lagunavicius, Zivile Kiveryte, Vilma Zimbaite-Ruskuliene, Tomas Radzvilavicius, Arvydas Janulaitis | Duality of polynucleotide substrates ... | RNase H,All properties |
| 2008 | Gemma Serrano-Heras, Alicia Bravo, Margarita Salas | Phage phi29 protein p56 prevents vira... | |
| 2008 | Linda J Reha-Krantz | Recent patents of gene sequences rela... | |
| 2007 | Patricia Pérez-Arnaiz, Elisa Longás, Laurentino Villar, José María Lázaro, Margarita Salas, Miguel de Vega | Involvement of phage phi29 DNA polyme... | |
| 2007 | Andrea J Berman, Satwik Kamtekar, Jessica L Goodman, José María Lázaro, Miguel de Vega, Luis Blanco, Margarita Salas, Thomas A Steitz | Structures of phi29 DNA polymerase co... | |
| 2006 | Satwik Kamtekar, Andrea J Berman, Jianhong Wang, José María Lázaro, Miguel de Vega, Luis Blanco, Margarita Salas, Thomas A Steitz | The phi29 DNA polymerase:protein-prim... | |
| 2006 | Patricia Pérez-Arnaiz, José María Lázaro, Margarita Salas, Miguel de Vega | Involvement of phi29 DNA polymerase t... | |
| 2006 | Elisa Longás, Miguel de Vega, José María Lázaro, Margarita Salas | Functional characterization of highly... | Cloned or native,Full length or truncated,3-5' exo,Vmax,All properties |
| 2006 | Thomas A Steitz | Visualizing polynucleotide polymerase... | |
| 2005 | Clyde A Hutchison, Hamilton O Smith, Cynthia Pfannkoch, J Craig Venter | Cell-free cloning using phi29 DNA pol... | |
| 2005 | Irene Rodríguez, José María Lázaro, Luis Blanco, Satwik Kamtekar, Andrea J Berman, Jianhong Wang, Thomas A Steitz, Margarita Salas, Miguel de Vega | A specific subdomain in phi29 DNA pol... | Full length or truncated,Processivity,3-5' exo,All properties |
| 2005 | Juan Luis Asensio, Armando Albert, Daniel Muñoz-Espín, Carlos Gonzalez, Juan Hermoso, Laurentino Villar, Jesús Jiménez-Barbero, Margarita Salas, Wilfried J J Meijer | Structure of the functional domain of... | |
| 2005 | Jianhong Wang, Satwik Kamtekar, Andrea J Berman, Thomas A Steitz | Correction of X-ray intensities from ... | |
| 2005 | Vijay Gadkar, Matthias C Rillig | Application of Phi29 DNA polymerase m... | |
| 2005 | Masahiro Sato, Masato Ohtsuka, Yuhsuke Ohmi | Usefulness of repeated GenomiPhi, a p... | |
| 2005 | Naoyuki Umetani, Michiel F G de Maat, Takuji Mori, Hiroya Takeuchi, Dave S B Hoon | Synthesis of universal unmethylated c... | |
| 2005 | Verónica Truniger, Ana Bonnin, José María Lázaro, Miguel de Vega, Margarita Salas | Involvement of the "linker" region be... | 3-5' exo,5-3' exo,All properties |
| 2004 | E Adachi, K Shimamura, S Wakamatsu, H Kodama | Amplification of plant genomic DNA by... | |
| 2004 | Irene Rodríguez, José María Lázaro, Margarita Salas, Miguel de Vega | phi29 DNA polymerase-terminal protein... | |
| 2004 | Víctor González-Huici, Martín Alcorlo, Margarita Salas, José M Hermoso | Binding of phage Phi29 architectural ... | |
| 2004 | C X Wang, E Zakharova, Jinming Li, Catherine M Joyce, Jianhong Wang, William H Konigsberg | Pre-steady-state kinetics of RB69 DNA... | |
| 2004 | Satwik Kamtekar, Andrea J Berman, Jianhong Wang, José María Lázaro, Miguel de Vega, Luis Blanco, Margarita Salas, Thomas A Steitz | Insights into strand displacement and... | Processivity,All properties |
| 2004 | Verónica Truniger, José María Lázaro, Margarita Salas | Function of the C-terminus of phi29 D... | |
| 2004 | Verónica Truniger, José María Lázaro, Margarita Salas | Two positively charged residues of ph... | |
| 2004 | Masahiro Sato, Masato Ohtsuka, Yuhsuke Ohmi | Repeated GenomiPhi, phi29 DNA polymer... | |
| 2003 | Verónica Truniger, José María Lázaro, Miguel de Vega, Luis Blanco, Margarita Salas | phi 29 DNA polymerase residue Leu384,... | |
| 2003 | Irene Rodríguez, José María Lázaro, Margarita Salas, Miguel de Vega | phi29 DNA polymerase residue Phe128 o... | |
| 2002 | Irene Gascón, José L Carrascosa, Laurentino Villar, José María Lázaro, Margarita Salas | Importance of the N-terminal region o... | |
| 2002 | Verónica Truniger, José María Lázaro, Francisco J Esteban, Luis Blanco, Margarita Salas | A positively charged residue of phi29... | |
| 2002 | Ralf Eisenbrandt, José María Lázaro, Margarita Salas, Miguel de Vega | Phi29 DNA polymerase residues Tyr59, ... | 3-5' exo,5-3' exo,All properties |
| 2002 | Verónica Truniger, José María Lázaro, Luis Blanco, Margarita Salas | A highly conserved lysine residue in ... | |
| 2002 | John R Nelson, Yuyang Christine Cai, Theresa L Giesler, Joseph W Farchaus, Shanmuuga T Sundaram, Maria Ortiz-Rivera, Lou P Hosta, Peter L Hewitt, J Anthony Mamone, C Palaniappan, Carl W Fuller | TempliPhi, phi29 DNA polymerase based... | Cloned or native,Tagged,Full length or truncated,Processivity,3-5' exo,Maximum Product Length,Vmax,Overall Error Rate,All properties |
| 2001 | F B Dean, John R Nelson, Theresa L Giesler, R S Lasken | Rapid amplification of plasmid and ph... | |
| 2001 | Wilfried J J Meijer, A Serna-Rico, Margarita Salas | Characterization of the bacteriophage... | |
| 2001 | Arjan B Brenkman, M R Heideman, Verónica Truniger, Margarita Salas, Peter C van der Vliet | The (I/Y)XGG motif of adenovirus DNA ... | |
| 2001 | Wilfried J J Meijer, J A Horcajadas, Margarita Salas | Phi29 family of phages. | |
| 2000 | V Gonzalez-Huici, José María Lázaro, Margarita Salas, José M Hermoso | Specific recognition of parental term... | |
| 2000 | Emmanuelle Dufour, Juan Méndez, José María Lázaro, Miguel de Vega, Luis Blanco, Margarita Salas | An aspartic acid residue in TPR-1, a ... | |
| 2000 | Miguel de Vega, José María Lázaro, Margarita Salas | Phage phi 29 DNA polymerase residues ... | |
| 2000 | Verónica Truniger, Luis Blanco, Margarita Salas | Analysis of O29 DNA polymerase by par... | |
| 2000 | A Serna-Rico, B Illana, Margarita Salas, Wilfried J J Meijer | The putative coiled coil domain of th... | |
| 2000 | Alicia Bravo, B Illana, Margarita Salas | Compartmentalization of phage phi29 D... | |
| 2000 | Irene Gascón, José María Lázaro, Margarita Salas | Differential functional behavior of v... | |
| 1999 | Ana Bonnin, José María Lázaro, Luis Blanco, Margarita Salas | A single tyrosine prevents insertion ... | |
| 1999 | Miguel de Vega, Luis Blanco, Margarita Salas | Processive proofreading and the spati... | Cloned or native,Full length or truncated,3-5' exo,5-3' exo,Vmax,All properties |
| 1999 | D Canceill, Enrique Viguera, S Dusko Ehrlich | Replication slippage of different DNA... | |
| 1999 | M Elías-Arnanz, Margarita Salas | Resolution of head-on collisions betw... | |
| 1999 | A M Abril, S Marco, José L Carrascosa, Margarita Salas, José M Hermoso | Oligomeric structures of the phage ph... | |
| 1999 | B Illana, José María Lázaro, C Gutiérrez, Wilfried J J Meijer, Luis Blanco, Margarita Salas | Phage phi29 terminal protein residues... | |
| 1999 | Verónica Truniger, Luis Blanco, Margarita Salas | Role of the "YxGG/A" motif of Phi29 D... | |
| 1998 | V Murthy, Wilfried J J Meijer, Luis Blanco, Margarita Salas | DNA polymerase template switching at ... | |
| 1998 | Miguel de Vega, José María Lázaro, Margarita Salas, Luis Blanco | Mutational analysis of phi29 DNA poly... | |
| 1998 | P Crucitti, José María Lázaro, V Benes, Margarita Salas | Bacteriophage phi29 early protein p17... | |
| 1998 | Miguel de Vega, Luis Blanco, Margarita Salas | phi29 DNA polymerase residue Ser122, ... | |
| 1998 | Verónica Truniger, José María Lázaro, Margarita Salas, Luis Blanco | Phi 29 DNA polymerase requires the N-... | |
| 1998 | B Illana, A Zaballos, Luis Blanco, Margarita Salas | The RGD sequence in phage phi29 termi... | |
| 1998 | J Saturno, José María Lázaro, Luis Blanco, Margarita Salas | Role of the first aspartate residue o... | |
| 1997 | J Mendez, Luis Blanco, Margarita Salas | Protein-primed DNA replication: a tra... | |
| 1997 | Alicia Bravo, Margarita Salas | Initiation of bacteriophage phi29 DNA... | |
| 1997 | J A Esteban, Luis Blanco, Laurentino Villar, Margarita Salas | In vitro evolution of terminal protei... | |
| 1997 | A M Abril, Margarita Salas, J M Andreu, José M Hermoso, G Rivas | Phage phi29 protein p6 is in a monome... | |
| 1997 | M Elías-Arnanz, Margarita Salas | Bacteriophage phi29 DNA replication a... | |
| 1997 | J Saturno, José María Lázaro, Francisco J Esteban, Luis Blanco, Margarita Salas | o29 DNA polymerase residue Lys383, in... | |
| 1997 | M S Soengas, C R Mateo, G Rivas, Margarita Salas, A U Acuña, C Gutiérrez | Structural features of phi29 single-s... | |
| 1996 | R Freire, M Serrano, Margarita Salas, José M Hermoso | Activation of replication origins in ... | |
| 1996 | B Illana, Luis Blanco, Margarita Salas | Functional characterization of the ge... | |
| 1996 | Luis Blanco, Margarita Salas | Relating structure to function in phi... | |
| 1996 | Miguel de Vega, J M Lazaro, Margarita Salas, Luis Blanco | Primer-terminus stabilization at the ... | |
| 1995 | José María Lázaro, Luis Blanco, Margarita Salas | Purification of bacteriophage phi 29 ... | |
| 1995 | Luis Blanco, Margarita Salas | Mutational analysis of bacteriophage ... | |
| 1995 | M A Blasco, Juan Méndez, José María Lázaro, Luis Blanco, Margarita Salas | Primer terminus stabilization at the ... | |
| 1995 | J Saturno, Luis Blanco, Margarita Salas, J A Esteban | A novel kinetic analysis to calculate... | |
| 1994 | J A Esteban, M S Soengas, Margarita Salas, Luis Blanco | 3'-->5' exonuclease active site of ph... | |
| 1994 | Luis Blanco, José María Lázaro, Miguel de Vega, Ana Bonnin, Margarita Salas | Terminal protein-primed DNA amplifica... | Maximum Product Length,All properties |
| 1993 | J A Esteban, Margarita Salas, Luis Blanco | Fidelity of phi 29 DNA polymerase. Co... | |
| 1993 | M A Blasco, José María Lázaro, Luis Blanco, Margarita Salas | Phi 29 DNA polymerase active site. Th... | |
| 1992 | DNA Replication | ||
| 1992 | M A Blasco, J A Esteban, Juan Méndez, Luis Blanco, Margarita Salas | Structural and functional studies on ... | |
| 1992 | J A Esteban, Antonio Bernad, Margarita Salas, Luis Blanco | Metal activation of synthetic and deg... | |
| 1992 | C Garmendia, Antonio Bernad, J A Esteban, Luis Blanco, Margarita Salas | The bacteriophage phi 29 DNA polymera... | Full length or truncated,3-5' exo,Molecular Weight,All properties |
| 1992 | M S Soengas, J A Esteban, José María Lázaro, Antonio Bernad, M A Blasco, Margarita Salas, Luis Blanco | Site-directed mutagenesis at the Exo ... | |
| 1992 | M A Blasco, José María Lázaro, Antonio Bernad, Luis Blanco, Margarita Salas | Phi 29 DNA polymerase active site. Mu... | |
| 1992 | Luis Blanco, Antonio Bernad, J A Esteban, Margarita Salas | DNA-independent deoxynucleotidylation... | |
| 1991 | M A Blasco, Antonio Bernad, Luis Blanco, Margarita Salas | Characterization and mapping of the p... | |
| 1990 | M A Blasco, Luis Blanco, E Parés, Margarita Salas, Antonio Bernad | Structural and functional analysis of... | |
| 1990 | Antonio Bernad, Luis Blanco, Margarita Salas | Site-directed mutagenesis of the YCDT... | |
| 1990 | Antonio Bernad, José María Lázaro, Margarita Salas, Luis Blanco | The highly conserved amino acid seque... | |
| 1990 | C Garmendia, José M Hermoso, Margarita Salas | Functional domain for priming activit... | |
| 1989 | Luis Blanco, Antonio Bernad, José María Lázaro, G Martín, C Garmendia, Margarita Salas | Highly efficient DNA synthesis by the... | Full length or truncated,Processivity,Molecular Weight,All properties |
| 1989 | A Zaballos, Margarita Salas | Functional domains in the bacteriopha... | |
| 1989 | A Zaballos, José María Lázaro, E Méndez, R P Mellado, Margarita Salas | Effects of internal deletions on the ... | |
| 1988 | A Zaballos, R P Mellado, Margarita Salas | Initiation of phage phi 29 DNA replic... | |
| 1988 | C Garmendia, Margarita Salas, José M Hermoso | Site-directed mutagenesis in the DNA ... | |
| 1987 | Luis Blanco, I Prieto, J Gutiérrez, Antonio Bernad, José María Lázaro, José M Hermoso, Margarita Salas | Effect of NH4+ ions on phi 29 DNA-pro... | |
| 1986 | A Zaballos, Margarita Salas, R P Mellado | Initiation of phage phi 29 DNA replic... | |
| 1986 | J Gutiérrez, J Vinós, I Prieto, E Méndez, José M Hermoso, Margarita Salas | Signals in the phi 29 DNA-terminal pr... | |
| 1986 | Luis Blanco, Margarita Salas | Effect of aphidicolin and nucleotide ... | |
| 1986 | P García, José M Hermoso, J A García, E García, R López, Margarita Salas | Formation of a covalent complex betwe... | |
| 1985 | Luis Blanco, Margarita Salas | Characterization of a 3'----5' exonuc... | |
| 1985 | José M Hermoso, E Méndez, F Soriano, Margarita Salas | Location of the serine residue involv... | |
| 1985 | Luis Blanco, Margarita Salas | Replication of phage phi 29 DNA with ... | |
| 1984 | Margarita Salas | A new mechanism for the initiation of... | |
| 1984 | I Prieto, José María Lázaro, J A García, José M Hermoso, Margarita Salas | Purification in a functional form of ... | |
| 1984 | Luis Blanco, Margarita Salas | Characterization and purification of ... | Cloned or native,Tagged,Full length or truncated,Molecular Weight,Other accessory protein(s),All properties |
| 1984 | M G Blanco, C Hardisson, J A Salas | Resistance in inhibitors of RNA polym... | |
| 1983 | R P Mellado, Margarita Salas | Initiation of phage phi 29 DNA replic... | |
| 1982 | M A Peñalva, Margarita Salas | Initiation of phage phi 29 DNA replic... | Cloned or native,Tagged,Other accessory protein(s),All properties |
| 1981 | S Krawiec, F Jiménez, J A García, N Villanueva, J Sogo, Margarita Salas | The orderly, in vitro emergence of DN... | |
| 1981 | José L Carrascosa, E Méndez, J Corral, V Rubio, G Ramírez, Margarita Salas, E Viñuela | Structural organization of Bacillus s... | |
| 1980 | J Kuznar, María L Salas, E Viñuela | DNA-dependent RNA polymerase in Afric... | |
| 1979 | J M Sogo, P Rodeño, T Koller, E Viñuela, Margarita Salas | Comparison of the A-T rich regions an... | |
| 1979 | J M Sogo, M R Inciarte, J Corral, E Viñuela, Margarita Salas | RNA polymerase binding sites and tran... | |
| 1978 | Margarita Salas, R P Mellado, E Viñuela | Characterization of a protein covalen... | |
| 1977 | Ana Camacho, F Jiménez, J De La Torre, José L Carrascosa, R P Mellado, C Vásquez, E Viñuela, Margarita Salas | Assembly of Bacillus subtilis phage p... | |
| 1976 | José L Carrascosa, Ana Camacho, F Moreno, F Jiménez, R P Mellado, E Viñuela, Margarita Salas | Bacillus subtilis phage phi29. Charac... | |
| 1976 | E Viñuela, Ana Camacho, F Jiménez, José L Carrascosa, G Ramírez, Margarita Salas | Structure and assembly of phage phi29. | |
| 1976 | M R Inciarte, E Viñuela, Margarita Salas | Transcription in vitro of phi29 DNA a... | |
| 1976 | M R Inciarte, José María Lázaro, Margarita Salas, E Vińuela | Physical map of bacteriophage phi29 DNA. | |
| 1975 | José L Carrascosa, F Jiménez, E Viñuela, Margarita Salas | Synthesis in vitro of phi29-specific ... | |
| 1975 | Ana Camacho, José L Carrascosa, E Vinuela, Margarita Salas | Discrepancy in the mobility of a prot... | |
| 1974 | F Jiménez, J Avila, E Viñuela, Margarita Salas | Initiation of the transcription of ph... | |
| 1974 | V Rubio, Margarita Salas, E Viñuela, P Usobiaga, J L Saiz, J F Llopis | Biophysical properties of bacteriopha... | |
| 1973 | José L Carrascosa, E Viñuela, Margarita Salas | Proteins induced in Bacillus subtilis... | |
| 1972 | G Ramírez, E Méndez, Margarita Salas, E Viñuela | Head-neck connecting protein in phage... | |
| 1972 | G Alvarez, E Salas, N Pérez, J E Celis | Phi29 bacteriophage structural proteins. | |
| 1972 | José M Hermoso, J Avila, F Jiménez, Margarita Salas | RNA polymerase from Bacillus amyloliq... | |
| 1972 | A Talavera, Margarita Salas, E Viñuela | Temperature-sensitive mutants affecte... | |
| 1971 | J Avila, José M Hermoso, E Vinuela, Margarita Salas | Purification and properties of DNA-de... | |
| 1970 | J Avila, José M Hermoso, E Viñuela, Margarita Salas | Subunit composition of B. subtilis RN... |
Protein sequence:
| Name | 3'-5' exo activity | 5'-3' exo activity | Frameshift Rate | Substitution Rate | General Error Rate |
|---|---|---|---|---|---|
| Phi29 D12AD66A | No (1) | ||||
| Phi29 S192N | |||||
| Phi29 K196R | |||||
| Phi29 K196I | |||||
| Phi29 F198Y | |||||
| Phi29 F198V | |||||
| Phi29 K206R | |||||
| phi29 V250F | |||||
| phi29 V250A | |||||
| phi29 Y254F | |||||
| Phi29 K260I | |||||
| phi29 Y390S | |||||
| phi29 Y390F | |||||
| phi29 T434N | Yes (1) | ||||
| phi29 A437G | Yes (1) | ||||
| phi29 R438I | Yes (1) | ||||
| phi29 R438K | Yes (1) | ||||
| phi29 (-TPR2) | Yes (1) | ||||
| phi29 el(398-420) |
Protein information: Uniprot P03680 search for Phi29 at UniProt
Sequence information: NCBI nucleotides European nucleotide archive