A homogeneous, high-throughput fluorescence resonance energy transfer-based DNA polymerase assay.

Shapiro A, Rivin O, Gao N, Hajec L
Anal Biochem (2005), Volume 347, Page 254
PubMed entry Publisher's site

Abstract:

A homogeneous, fluorescence resonance energy transfer (FRET)-based DNA ...
A homogeneous, fluorescence resonance energy transfer (FRET)-based DNA polymerase assay that is suitable for high-throughput screening for inhibitors, and can also be used for steady-state kinetic investigations, is described. The activity, kinetic mechanism, and processivity of the isolated alpha subunit of DNA polymerase III, the product of the dnaE gene, from the gram-negative pathogen Haemophilus influenzae were investigated using the FRET assay.

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