Crystallization and preliminary X-ray diffraction studies of DNA polymerase from the thermophilic archaeon Sulfolobus solfataricus.

Nastopoulos V, Pisani FM, Savino C, Federici L, Rossi M, Tsernoglou D
Acta Crystallogr D Biol Crystallogr (1998), Volume 54, Page 1002
PubMed entry

Abstract:

The thermophilic and thermostable family B DNA polymerase from the ...
The thermophilic and thermostable family B DNA polymerase from the archaeon Sulfolobus solfataricus (Mr of about 100 kDa) has been crystallized by the hanging-drop vapour-diffusion method at 294 K using ammonium sulfate as precipitant. The crystals belong to the monoclinic space group C2 with cell dimensions a = 187.4, b = 68.5, c = 125.8 A and beta = 107.8 degrees and diffract up to 2.7 A resolution on a rotating-anode X-ray source. Native data have been collected at 100 K. A heavy-atom derivative search is in progress.

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