Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase.

Polymerases:

Topics:

Mutational Analysis, Nucleotide Analogs / Template Lesions, Fidelity, Nucleotide Incorporation, Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 A89TD363N Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. Nucleotide Substitution Rate 68 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+1))
T4 A89TD363N Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. Nucleotide Substitution Rate 100 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+2))
T4 A89TD363N Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. Nucleotide Substitution Rate 62 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+3))
T4 A89TD363N Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. Nucleotide Substitution Rate 254 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+2))
T4 G694S Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. Nucleotide Substitution Rate 27 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+1))
T4 G694S Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. Nucleotide Substitution Rate 16 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+2))
T4 G694S Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. Nucleotide Substitution Rate 20 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+3))
T4 G694S Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. Nucleotide Substitution Rate 17 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay (Host: B40(sup+2))
T4 Q731S Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. Nucleotide Substitution Rate 0.001 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Q731Y Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. Nucleotide Substitution Rate 0.015 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 A89TD363N/Q731S Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. Nucleotide Substitution Rate 2.4 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 A89TD363N/Q731Y Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. Nucleotide Substitution Rate 15 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 G694SQ731S Studies on the biochemical basis of mutation. IV. Effect of amino acid substitution on the enzymatic and biological properties of bacteriophage T4 DNA polymerase. Nucleotide Substitution Rate 0.12 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay

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