Misincorporation of ribonucleotides by DNA polymerase during in vitro DNA replication.

Ide H, Yagi R, Yamaoka T, Kimura Y
Nucleic Acids Symp Ser (1993), Volume , Page 133
PubMed entry

Abstract:

The fidelity of DNA replication with respect to misincorporation of ...
The fidelity of DNA replication with respect to misincorporation of ribonucleotides into DNA has been studied. Primed M13 DNA was replicated by E. coli DNA polymerase I in the presence of four dNTPs, a single rNTP and Mg2+ as a cofactor. All four rNTPs were incorporated into DNA in place of the corresponding dNTPs when rNTP/dNTP ratio was 1000:1. The frequency of rNTP misincorporation was C > G > A > U.

Polymerases:

Eco Pol I

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