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Reference: The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination., Polymerase: RB69

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Polymerase Reference Property Result Context
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 909uM Reaction: Misincorporation; Substrate: dATP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 761uM Reaction: Misincorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 4500uM Reaction: Misincorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 725uM Reaction: Misincorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 3363uM Reaction: Misincorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 1715uM Reaction: Misincorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 2085uM Reaction: Misincorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 1986uM Reaction: Misincorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 1391uM Reaction: Misincorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 50uM Reaction: Nucleotide incorporation; Substrate: dATP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 69uM Reaction: Nucleotide incorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 26uM Reaction: Nucleotide incorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 42uM Reaction: Nucleotide incorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 811uM Reaction: Misincorporation; Substrate: dATP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 784uM Reaction: Misincorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 1800uM Reaction: Misincorporation; Substrate: dATP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.004 /second Reaction: Misincorporation; Substrate: dATP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.011 /second Reaction: Misincorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.77 /second Reaction: Misincorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.041 /second Reaction: Misincorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.001 /second Reaction: Misincorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.09 /second Reaction: Misincorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.013 /second Reaction: Misincorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.14 /second Reaction: Misincorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.015 /second Reaction: Misincorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 220 /second Reaction: Nucleotide incorporation; Substrate: dATP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 200 /second Reaction: Nucleotide incorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 167 /second Reaction: Nucleotide incorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 240 /second Reaction: Nucleotide incorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.013 /second Reaction: Misincorporation; Substrate: dATP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.003 /second Reaction: Misincorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.08 /second Reaction: Misincorporation; Substrate: dATP

Version:20241218

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.