show help

Results for:

Reference: The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination., Polymerase: RB69 L561A

Welcome to the results summary page

This page presents all the results for a polymerase, property or reference. To find more information about a polymerase or reference, follow the relevant link.

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

Polymerase Reference Property Result Context
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Frameshift Error Rate 1.2 errors/bp Technique: RB69 rII 131 reversion
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Frameshift Error Rate 4.5 errors/bp Technique: RB69 rII UV232 reversion
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Nucleotide Substitution Rate 2.6 Mutation frequency (relative to WT) Technique: RB69 rII UV256 reversion
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Nucleotide Substitution Rate 4.3 Mutation frequency (relative to WT) Technique: RB69 rII UV375 reversion
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 1467uM Reaction: Misincorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 17uM Reaction: Nucleotide incorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 28uM Reaction: Misincorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 51uM Reaction: Nucleotide incorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 205uM Reaction: Misincorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 155uM Reaction: Misincorporation; Substrate: dATP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 30uM Reaction: Nucleotide incorporation; Substrate: dATP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 228uM Reaction: Misincorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 368uM Reaction: Misincorporation; Substrate: dATP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 310uM Reaction: Misincorporation; Substrate: dATP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 161uM Reaction: Misincorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 537uM Reaction: Misincorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 251uM Reaction: Misincorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 2010uM Reaction: Misincorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 50uM Reaction: Nucleotide incorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 507uM Reaction: Misincorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.03 /second Reaction: Misincorporation; Substrate: dATP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.11 /second Reaction: Misincorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.26 /second Reaction: Misincorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.18 /second Reaction: Misincorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.03 /second Reaction: Misincorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.8 /second Reaction: Misincorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.14 /second Reaction: Misincorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.05 /second Reaction: Misincorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.13 /second Reaction: Misincorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 108 /second Reaction: Nucleotide incorporation; Substrate: dATP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 89 /second Reaction: Nucleotide incorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 101 /second Reaction: Nucleotide incorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 115 /second Reaction: Nucleotide incorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.03 /second Reaction: Misincorporation; Substrate: dATP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.01 /second Reaction: Misincorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.75 /second Reaction: Misincorporation; Substrate: dATP

Version:20241007

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.