Cloning and expression of T4 DNA polymerase.

Lin TC, Rush J, Spicer EK, Konigsberg WH
Proceedings of the National Academy of Sciences of the United States of America (1987), Volume 84, Page 7000
PubMed entry Full article text

Abstract:

The structural gene coding for bacteriophage T4 DNA polymerase (gene ...
The structural gene coding for bacteriophage T4 DNA polymerase (gene 43) has been cloned into inducible plasmid vectors, which provide a source for obtaining large amounts of this enzyme after induction. The T4 DNA polymerase produced in this fashion was purified by an innovative three-step procedure and was fully active.

Polymerases:

T4

Topics:

Biotech Applications, Methods

One line summary:

Three-step procedure for purifying fully active T4 polymerase

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