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Reference: Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal., Polymerase: Klenow fragment

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Polymerase Reference Property Result Context
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Polymerase Catalytic Residue Amino Acids Asp705, Asp882, and Glu883
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. 3-5' Exonuclease (proofreading) Yes
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Residues Involved in Catalysis of 3-5' Exo Asp355, Glu357, Asp424, and Asp501
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Processivity 70bp
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Specific Activity 5500 units/mg Technique: Polymerase Assay (M13 DNA)
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Specific Activity 9700 units/mg Technique: Polymerase Assay (calf thymus DNA)
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. KM 2.3uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. KM 1.8nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. kcat 5.2 /second Reaction: Nucleotide incorporation; Substrate: dNTPs

Using Polbase tables:

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Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

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It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.