BioRad iTaq DNA polymerase

From Unknown (limited name: BioRad iTaq DNA polymerase)
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Welcome to the Polymerase Page

This page presents all the information in Polbase for BioRad iTaq DNA polymerase.

Mutants:

At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.

Structures/Sequence:

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References:

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Results:

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BioRad iTaq DNA polymerase is a family A enzyme from Unknown.

iTaq is a Hot Start DNA polymerase available from BioRad with the catalog number 1708870.

 

PCR setup is as follows:

Component                     Volume per 50ul reaction                      Final Concentration

10X iTaq buffer                              5.0ul                                                      1X

50mM MgCl2                                  1.5ul                                                   1.5mM

10mM dNTP                                   1.0ul                                                200uM each

iTaq DNA polymerase                    0.25ul                                                    1.25U

Primers                                                                                            100nM-500nM each

DNA template

Water                                         to 50ul

 

Recommended Cycling Conditions are as follows:

Polymerase activation:          95oC, 3min

25-40 cycles of:                   95oC, 30sec

                                          55oC, 30sec

                                          72oC, 1min

Hold at 4oC

Selected Properties for BioRad iTaq DNA polymerase:

 3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
no data no data no data no data no data

Version:20241007

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