Invitrogen Platinum II Taq Hot Start DNA polymerase

From Unknown (limited name: Invitrogen Platinum II Taq Hot Start DNA polymerase)
show help

Welcome to the Polymerase Page

This page presents all the information in Polbase for Invitrogen Platinum II Taq Hot Start DNA polymerase.

Mutants:

At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.

Structures/Sequence:

If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

External links:

Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.

References:

All references that refer to this Polymerase are listed with their topics and paper's title in the References table.

Results:

All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

Invitrogen Platinum II Taq Hot Start DNA polymerase is a family A enzyme from Unknown.

Platinum II Taq Hot Start DNA polymerase is available from Thermofisher with the catalog number 14966001.

 

Recommended PCR setup:

Component                                       Volume per 50ul reaction                 Final Concentration

5X Platinum II PCR Buffer                                   10ul                                                1X

10nM dNTP                                                         1ul                                         0.2mM each

Platinum II Taq Hot Start Polymerase                 0.4ul                                           0.04 U/ul

Platinum GC enhancer (optional)*                       10ul                                                1X

nuclease-free water to 50ul

 

*GC enhancer is recommended with targets with >65% GC sequences

 

 

Recommened Cycling Conditions:

initial denaturation              94oC, 2min

25-35 cycles of:                 94oC, 15sec

                                        60oC, 15sec*

                                        68oC, 15sec/kb*

4oC hold

 

*Steps can be combined to be 60oC for 15 seconds

Selected Properties for Invitrogen Platinum II Taq Hot Start DNA polymerase:

 3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
no data no data no data no data no data

Version:20241218

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.