Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases.

Brutlag D, Kornberg A
The Journal of biological chemistry (1972), Volume 247, Page 241
PubMed entry

Polymerases:

T4 A89TD363N,Klenow fragment,T4,Eco Pol I

Topics:

Kinetic Parameters, Fidelity, Exonuclease Activity

One line summary:

Function of 3'->5' exonuclease activity of DNA polymerases is to remove mispaired nucleotides which have been incorrectly incorporated.

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. Vmax 1100 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: pH (pH 7), Temp (37°C)
T4 Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. Vmax 350 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: pH (pH 7), Temp (37°C)
T4 Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. Vmax 850 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: pH (pH 7), Temp (30°C)
T4 Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. Vmax 27 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: pH (pH 7), Temp (30°C)
T4 A89TD363N Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. Vmax 790 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C)
T4 A89TD363N Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. Vmax 11 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (30°C)
Klenow fragment Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. Vmax 19 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (37°C)
Klenow fragment Enzymatic synthesis of deoxyribonucleic acid. 36. A proofreading function for the 3' leads to 5' exonuclease activity in deoxyribonucleic acid polymerases. Vmax 4.8 /minute Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Temp (37°C)
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