ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI.

Richardson CC, SCHILDKRAUT CL, APOSHIAN HV, Kornberg A
The Journal of biological chemistry (1964), Volume 239, Page 222
PubMed entry

Polymerases:

Eco Pol I Exonuclease II,Eco Pol I,Bsu,Eco Pol I*,Eco Pol IV,Eco Pol V

Topics:

Historical Protein Properties (MW, pI, ...), Modulators/Inhibitors, Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol I ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. 3-5' Exonuclease (proofreading) Unspecified
Eco Pol I ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. 5-3' Exonuclease Unspecified
Eco Pol I ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. Optimal pH pH 7.4
Eco Pol I ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. Specific Activity 1.88E+04 units/mg Technique: Polymerase Assay (calf thymus DNA)
Eco Pol I ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. Percent/Fold Effect 30 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: pH (pH 7)
Eco Pol I ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. Percent/Fold Effect 30 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: pH (pH 7.8)
Eco Pol I Exonuclease II ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. 3-5' Exonuclease (proofreading) Yes
Eco Pol I Exonuclease II ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. Optimal pH pH 9
Bsu ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. 3-5' Exonuclease (proofreading) No
Bsu ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. 5-3' Exonuclease No
Bsu ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. Optimal pH pH 7.4
Bsu ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. Percent/Fold Effect 0 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Mg++ (7mM)
Bsu ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. Percent/Fold Effect 70 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Mg++ (.33mM)
Bsu ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. Percent/Fold Effect 50 % decrease Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Mg++ (33mM)
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Contributor: Joshua Mazzola

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