Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break.

Kelly RB, Cozzarelli NR, Deutscher MP, Lehman IR, Kornberg A
The Journal of biological chemistry (1970), Volume 245, Page 39
PubMed entry

Polymerases:

Eco Pol I,T4

Topics:

Historical Protein Properties (MW, pI, ...), Nucleotide Incorporation, Exonuclease Activity, Nick Extension

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. 5-3' Exonuclease No
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. 3-5' Exonuclease (proofreading) Yes
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. 5-3' Exonuclease Yes
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. Optimal pH pH 7.4
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. Specific Activity 1.8E+04 units/mg
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. Nick Extension Yes, via exo activity
Eco Pol I Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of duplex deoxyribonucleic acid by polymerase at a single strand break. Strand Displacement Yes
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Contributor: Joshua Mazzola

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