Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.

Setlow P, Brutlag D, Kornberg A

The Journal of biological chemistry (1972), Volume 247, Page 224

PubMed entry

Polymerases:

Eco Pol I,Eco Pol I - Small Fragment,Klenow fragment

Topics:

Historical Protein Properties (MW, pI, ...), Exonuclease Activity

Status:

Results:

Polymerase	Reference	Property	Result	Context
Eco Pol I - Small Fragment	Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.	Molecular Weight	3.6E+04 Dalton
Eco Pol I - Small Fragment	Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.	3-5' Exonuclease (proofreading)	No
Eco Pol I - Small Fragment	Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.	5-3' Exonuclease	Yes
Eco Pol I	Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.	Molecular Weight	1.09E+05 Dalton
Eco Pol I	Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.	3-5' Exonuclease (proofreading)	Yes
Eco Pol I	Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.	5-3' Exonuclease	Yes
Eco Pol I	Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.	Specific Activity	4500 units/mg	Technique: Polymerase Assay (calf thymus DNA)
Eco Pol I	Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.	Extinction Coefficient	0.85 at 278nm
Klenow fragment	Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.	Molecular Weight	7.6E+04 Dalton	Technique: SDS-acrylamide gel electrophoresis
Klenow fragment	Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.	3-5' Exonuclease (proofreading)	Yes
Klenow fragment	Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.	5-3' Exonuclease	No
Klenow fragment	Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.	Specific Activity	1.534E+04 units/mg	Technique: Polymerase Assay (calf thymus DNA)
Klenow fragment	Deoxyribonucleic acid polymerase: two distinct enzymes in one polypeptide. I. A proteolytic fragment containing the polymerase and 3' leads to 5' exonuclease functions.	Extinction Coefficient	0.93 at 278nm

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