Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4.

Goulian M, Lucas ZJ, Kornberg A
The Journal of biological chemistry (1968), Volume 243, Page 627
PubMed entry

Polymerases:

T4

Topics:

Historical Protein Properties (MW, pI, ...), Modulators/Inhibitors, Nucleotide Incorporation, Exonuclease Activity, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. Molecular Weight 1.12E+05 Dalton
T4 Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. 3-5' Exonuclease (proofreading) Yes
T4 Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. 5-3' Exonuclease No
T4 Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. Full length or truncated Full length
T4 Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. Optimal pH pH 8.5
T4 Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. Specific Activity 3.3E+04 units/mg Technique: Polymerase Assay (calf thymus DNA)
T4 Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. Strand Displacement No
T4 Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. Percent/Fold Effect 0 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Mg++ (6mM)
T4 Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. Percent/Fold Effect 75 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: Mg++ (.1mM)
T4 Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. Percent/Fold Effect 0 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: NTPs (0mM)
T4 Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. Percent/Fold Effect 49 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: GTP (0.2mM), ATP (0mM), CTP (0mM), TTP (0mM)
T4 Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. Percent/Fold Effect 81 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: GTP (0.2mM), ATP (0.2mM), CTP (0mM)
T4 Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. Percent/Fold Effect 96 % decrease Reaction: 3-5' Exonuclease; Substrate: DNA template; Experimental conditions: GTP (0.2mM), ATP (0.2mM), CTP (0.2mM), TTP (0mM)
T4 Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4. Gap Filling Yes
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