Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22.

Nossal NG, Hershfield MS
The Journal of biological chemistry (1971), Volume 246, Page 5414
PubMed entry

Polymerases:

T4,T4 Q731am

Topics:

Kinetic Parameters, Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22. KM 0.04uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22. KM 0.08uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22. Vmax 91 /minute Reaction: 3-5' Exonuclease
T4 Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22. Vmax 2.2 /minute Reaction: 3-5' Exonuclease; Technique: Steady State (substrate: E. coli DNA, heat denatured)
T4 Q731am Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22. KM 0.38uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Q731am Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22. KM 0.71uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Q731am Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22. Vmax 83 /minute Reaction: 3-5' Exonuclease
T4 Q731am Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22. Vmax 1.1 /minute Reaction: 3-5' Exonuclease
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