Adriamycin interactions with T4 DNA polymerase. Two modes of template-mediated inhibition.
Abstract:
We examined the effect of adriamycin on kinetics of DNA synthesis catalyzed by DNA polymerase purified from bacteriophage T4-infected Escherichia coli. Two distinct modes of enzyme inhibition occur: uncompetitive and competitive at "low" and "high" drug:DNA nucleotide molar ratios, respectively. Competitive inhibition is not observed unless an unblocked amino group is present on the sugar (daunosamine) moiety. A model is proposed to relate the enzyme inhibition kinetics to intercalative and ionic binding of adriamycin to DNA.
Polymerases:
Topics:
Mutational Analysis, Enzyme Substrate Interactions
One line summary:
Adriamycin and Daunorubicin binding to DNA have varying effects on the rate of DNA replication by the T4 DNA polymerase
Status:
| new | topics/pols set | partial results | complete | validated |
Results:
No results available for this paper.