On the fidelity of DNA replication. The accuracy of T4 DNA polymerases in copying phi X174 DNA in vitro.

Kunkel TA, Loeb LA, Goodman MF
The Journal of biological chemistry (1984), Volume 259, Page 1539
PubMed entry

Polymerases:

T4,Eco Pol I,T4 A737V,T4 A89TD363N

Topics:

Fidelity, Accessory Proteins/Complexes, Nucleotide Incorporation

One line summary:

the action of replication fidelity may be dependent partially on interaction with accessory proteins and complexes

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 On the fidelity of DNA replication. The accuracy of T4 DNA polymerases in copying phi X174 DNA in vitro. Nucleotide Substitution Rate 1E-06 errors/bp Technique: Reversion (1 mM dNTP)
T4 On the fidelity of DNA replication. The accuracy of T4 DNA polymerases in copying phi X174 DNA in vitro. Processivity 1840bp
Eco Pol I On the fidelity of DNA replication. The accuracy of T4 DNA polymerases in copying phi X174 DNA in vitro. Nucleotide Substitution Rate 9.01E-06 errors/bp Technique: Reversion (1 mM dNTP)
Eco Pol I On the fidelity of DNA replication. The accuracy of T4 DNA polymerases in copying phi X174 DNA in vitro. Processivity 3040bp
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