Klenow fragment
From
E. coli
(aliases:Klenow fragment, KF, Klenow fragment of E. coli DNA polymerase I, Klenow fragment of Pol I, Klenow, Pol Ik, Pol I(KF))
Welcome to the Polymerase Page
This page presents all the information in Polbase for Klenow fragment.
Mutants:At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.
Structures/Sequence:If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.
External links:Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.
References:All references that refer to this Polymerase are listed with their topics and paper's title in the References table.
Results:All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.
Help icons:
The symbol in the upper-right corner of the page
links to this help text. The question mark icon is used everywhere to indicate that help is available.
Klenow fragment
is a family A enzyme
from
E. coli.
Original enzyme used in PCR (since replaced by thermostable enzymes like Taq)
Original enzyme used in chain-terminator sequencing (Sanger)
First DNA polymerase crystal structure
Originally produced by protease digestion of DNA Pol I; subsequently made from a cloned construct.
Many of the papers in the collection describe studies using exo-minus Klenow fragment. No attempt has been made to separate these out.
Selected Properties for Klenow fragment: |
3'-5' exo activity | 5'-3' exo activity | Frameshift Rate | Substitution Rate | General Error Rate |
|---|---|---|---|---|---|
| Yes (16) | No (8) | 6.0e-07-1.0e-02 errors/bp (3) | 5.8e-06-2.8e-03 errors/bp (6) | 4.0e-05-5.2e-03 errors/bp (6) |
- References (185)
- Structures (11)
- Sequences (0)
- Mutants (13)...
- Formulations (0)
- Polymerase Lots (0)
- External links
| Year | Authors | Title | Results by property |
|---|---|---|---|
| 2013 | Leena Maddukuri, Sarah C Shuck, Robert Lawton Eoff, Linlin Zhao, Carmelo J Rizzo, F Peter Guengerich, Lawrence J Marnett | Replication, Repair and Translesion P... | |
| 2013 | Junpei Yamamoto, Tomoko Oyama, Tomohiro Kunishi, Chikahide Masutani, Fumio Hanaoka, Shigenori Iwai | A cyclobutane thymine-N4-methylcytosi... | |
| 2013 | Hongyun Wang, Nicholas Hurt, William B Dunbar | Measuring and modeling the kinetics o... | |
| 2013 | Ping Xie | Dynamics of DNA polymerase I (Klenow ... | |
| 2012 | Prakasha Gowda A S, Gowdahalli Krishnegowda, Zucai Suo, Shantu G Amin, Thomas E Spratt | Low fidelity bypass of O2-... | |
| 2012 | Sarah E Graham, FatimaSultana Syeda, G Andrés Cisneros | Computational prediction of residues ... | |
| 2011 | Oya Bermek, Nigel D F Grindley, Catherine M Joyce | Distinct roles of the active-site Mg2... | |
| 2011 | Daniel R Garalde, Christopher A Simon, Joseph M Dahl, Hongyun Wang, Mark Akeson, Kate R Lieberman | Distinct complexes of DNA polymerase ... | |
| 2011 | M Hogg, AE Sauer-Eriksson, Erik Johansson | Promiscuous DNA synthesis by human DN... | |
| 2011 | Chen Song, Chen Zhang, Meiping Zhao | Rapid and sensitive detection of DNA ... | |
| 2010 | Linda J Reha-Krantz | DNA polymerase proofreading: Multiple... | |
| 2010 | Ken Hirano, Yuichiro Yoshida, Tomomi Ishido, Yukihisa Wada, Naoji Moriya, Naoshi Yamazaki, Yoshiyuki Mizushina, Yoshinobu Baba, Mitsuru Ishikawa | Consecutive incorporation of fluoroph... | |
| 2010 | Guojie Zhao, Yifu Guan | Polymerization behavior of Klenow fra... | |
| 2009 | Md Monsur Ali, Shuhei Imoto, Yingqian Li, Shigeki Sasaki, Fumi Nagatsugi | Incorporation of an inducible nucleot... | |
| 2009 | Gudrun Stengel, Byron W Purse, L Marcus Wilhelmsson, Milan Urban, Robert D Kuchta | Ambivalent incorporation of the fluor... | |
| 2009 | Noriaki Minakawa, Shintaro Ogata, Mayumi Takahashi, Akira Matsuda | Selective recognition of unnatural im... | |
| 2009 | Brett Gyarfas, Felix Olasagasti, Seico Benner, Daniel R Garalde, Kate R Lieberman, Mark Akeson | Mapping the position of DNA polymeras... | |
| 2009 | Peter Sandin, Gudrun Stengel, Thomas Ljungdahl, Karl Börjesson, Bertil Macao, L Marcus Wilhelmsson | Highly efficient incorporation of the... | Vmax,Methods Featured,Incorporation of non-standard nucleotides,All properties |
| 2009 | Younguk Sun, Kalvin J Gregory, Val Golovlev | Efficiency and specificity of microRN... | |
| 2008 | Asim Sheriff, Edward Motea, Irene Lee, Anthony J Berdis | Mechanism and dynamics of translesion... | |
| 2008 | Pinky Kukreti, Kamalendra Singh, Amit Ketkar, Mukund J Modak | Identification of a new motif require... | 3-5' exo,5-3' exo,All properties |
| 2008 | Linda J Reha-Krantz | Recent patents of gene sequences rela... | |
| 2008 | Ravindra Venkatramani, Ravi Radhakrishnan | Effect of oxidatively damaged DNA on ... | |
| 2008 | Catherine M Joyce, Olga Potapova, Angela M DeLucia, Xuanwei Huang, Vandana Purohit Basu, Nigel D F Grindley | Fingers-closing and other rapid confo... | Cloned or native,Tagged,Tag Name,Full length or truncated,kcat,Methods Featured,All properties |
| 2008 | Janina Cramer, Gopinath Rangam, Andreas Marx, Tobias Restle | Varied active-site constraints in the... | |
| 2007 | Michael F Bailey, Edwin J C Van der Schans, David P Millar | Dimerization of the Klenow fragment o... | |
| 2007 | Yasushi Shigemori, Michio Oishi | Specific terminal labeling of DNA mol... | |
| 2007 | Flavia Barone, Scott D McCulloch, Peter Macpherson, Giovanni Maga, Masami Yamada, Takehiko Nohmi, Anna Minoprio, Filomena Mazzei, Thomas A Kunkel, Peter Karran, Margherita Bignami | Replication of 2-hydroxyadenine-conta... | |
| 2007 | Adam P Silverman, Qingfei Jiang, Myron F Goodman, Eric T Kool | Steric and electrostatic effects in D... | |
| 2007 | Gudrun Stengel, JP Gill, Peter Sandin, L Marcus Wilhelmsson, Bo Albinsson, Bengt Nordén, David P Millar | Conformational dynamics of DNA polyme... | |
| 2006 | Irene Lee, Anthony J Berdis | Fluorescent analysis of translesion D... | |
| 2006 | Shin Mizukami, Tae Woo Kim, Sandra A Helquist, Eric T Kool | Varying DNA base-pair size in subangs... | |
| 2006 | Ichiro Hirao, Michiko Kimoto, Tsuneo Mitsui, Tsuyoshi Fujiwara, Rie Kawai, Akira Sato, Yoko Harada, Shigeyuki Yokoyama | An unnatural hydrophobic base pair sy... | |
| 2006 | Changbei Ma, Zhiwen Tang, Kemin Wang, Weihong Tan, Jun Li, Wei Li, Zhongjie Li, Xiaohai Yang, Huimin Li, Lingfeng Liu | Real-time monitoring of DNA polymeras... | |
| 2005 | Alexander B Kotlyar, Natalia Borovok, Tatiana Molotsky, Dmitry Klinov, Benjamin Dwir, Eli Kapon | Synthesis of novel poly(dG)-poly(dG)-... | 3-5' exo,5-3' exo,All properties |
| 2005 | Kamalendra Singh, Mukund J Modak | Contribution of polar residues of the... | 3-5' exo,5-3' exo,All properties |
| 2005 | XinJing Tang, Julia L Richards, Adam E Peritz, Ivan J Dmochowski | Photoregulation of DNA polymerase I (... | |
| 2005 | Alexander B Kotlyar, Natalia Borovok, Tatiana Molotsky, Ludmila Fadeev, Michael Gozin | In vitro synthesis of uniform poly(dG... | 3-5' exo,5-3' exo,All properties |
| 2005 | Kristi Kincaid, Jeff W Beckman, Aleksandra Zivkovic, Randall L Halcomb, Joachim W Engels, Robert D Kuchta | Exploration of factors driving incorp... | |
| 2005 | JP Gill, Louis J Romano | Mechanism for N-acetyl-2-aminofluoren... | |
| 2004 | Kazuki Tainaka, Akimitsu Okamoto, Isao Saito | Development of a novel solvatochromic... | |
| 2004 | Muhammed F Hashim, James N Riggins, Nathalie Schnetz-Boutaud, Markus W Voehler, Michael P Stone, Lawrence J Marnett | In vitro bypass of malondialdehyde-de... | |
| 2004 | Michael B Doughty, Karam Aboudehen, Garland Anderson, Ke Li, Bob Moore, Tina Poolson | Side-chain conformational restriction... | |
| 2004 | Aviva S Meyer, Maureen Blandino, Thomas E Spratt | Escherichia coli DNA polymerase I (Kl... | |
| 2004 | C X Wang, E Zakharova, Jinming Li, Catherine M Joyce, Jianhong Wang, William H Konigsberg | Pre-steady-state kinetics of RB69 DNA... | kcat,All properties |
| 2003 | Vandana Purohit, Nigel D F Grindley, Catherine M Joyce | Use of 2-aminopurine fluorescence to ... | |
| 2003 | Lisa S Chen, Michael H Bahr, Terry L Sheppard | Effects of 8-chlorodeoxyadenosine on ... | |
| 2003 | Aashish Srivastava, Kamalendra Singh, Mukund J Modak | Phe 771 of Escherichia coli DNA polym... | |
| 2003 | Kamalendra Singh, Mukund J Modak | Presence of 18-A long hydrogen bond t... | |
| 2003 | Alexander Krieg, Stephan Laib, Thomas Ruckstuhl, Stefan Seeger | Real-time detection of nucleotide inc... | |
| 2003 | Robert M Turner, Nigel D F Grindley, Catherine M Joyce | Interaction of DNA polymerase I (Klen... | |
| 2003 | Victoria H Harris, Clifford L Smith, W Jonathan Cummins, Alan L Hamilton, David P Hornby, David M Williams | Recognition of base-pairing by DNA po... | |
| 2003 | Molly Chiaramonte, Chad L Moore, Kristi Kincaid, Robert D Kuchta | Facile polymerization of dNTPs bearin... | |
| 2003 | Olga Kornyushyna, Cynthia J Burrows | Effect of the oxidized guanosine lesi... | |
| 2002 | Olga Kornyushyna, Aym M Berges, James G Muller, Cynthia J Burrows | In vitro nucleotide misinsertion oppo... | |
| 2002 | Elizabeth H Z Thompson, Michael F Bailey, Edwin J C Van der Schans, Catherine M Joyce, David P Millar | Determinants of DNA mismatch recognit... | |
| 2002 | Vineeta Khare, Kristin A Eckert | The proofreading 3'-->5' exonuclease ... | 3-5' exo,5-3' exo,All properties |
| 2002 | Yuriy O Alekseyev, Louis J Romano | Effects of benzo[a]pyrene adduct ster... | |
| 2002 | Olga Potapova, Nigel D F Grindley, Catherine M Joyce | The mutational specificity of the Dbh... | Overall Error Rate,All properties |
| 2002 | Rajiv Gangurde, Mukund J Modak | Participation of active-site carboxyl... | |
| 2002 | Wai-Chung Lam, Elizabeth H Z Thompson, Olga Potapova, Xiaojun Chen Sun, Catherine M Joyce, David P Millar | 3'-5' exonuclease of Klenow fragment:... | 3-5' exo,5-3' exo,All properties |
| 2001 | Emmanuelle Delagoutte, Peter H von Hippel | Molecular mechanisms of the functiona... | |
| 2001 | I Terashima, Toshiro Matsuda, T W Fang, Naomi Suzuki, J Kobayashi, K Kohda, Shinya Shibutani | Miscoding potential of the N2-ethyl-2... | 3-5' exo,5-3' exo,All properties |
| 2001 | Holly H Hogrefe, J Cline, A E Lovejoy, K B Nielson | DNA polymerases from hyperthermophiles. | RT,Specific Activity,Processivity,Strand Displacement,3-5' exo,5-3' exo,KM,kcat,3-5' Exo Specific Activity,All properties |
| 2001 | P Zhuang, Alexander Kolbanovskiy, Shantu Amin, Nicholas E Geacintov | Base sequence dependence of in vitro ... | |
| 2001 | Manabu Yasui, S Matsui, M Ihara, Y R Laxmi, Shinya Shibutani, Toshiro Matsuda | Translesional synthesis on a DNA temp... | |
| 2001 | Thomas E Spratt | Identification of hydrogen bonds betw... | |
| 2001 | Yuriy O Alekseyev, Leonid Dzantiev, Louis J Romano | Effects of benzo[a]pyrene DNA adducts... | |
| 2001 | T C Lin, C X Wang, Catherine M Joyce, William H Konigsberg | 3'-5' Exonucleolytic activity of DNA ... | |
| 2000 | J C Morales, Eric T Kool | Importance of terminal base pair hydr... | |
| 2000 | D S Johnston, Michael P Stone | Replication of a site-specific trans-... | |
| 2000 | Yuriy O Alekseyev, Louis J Romano | In vitro replication of primer-templa... | |
| 2000 | Steven Tuske, Kamalendra Singh, Neerja Kaushik, Mukund J Modak | The J-helix of Escherichia coli DNA p... | |
| 2000 | Rajiv Gangurde, Neerja Kaushik, Kamalendra Singh, Mukund J Modak | A carboxylate triad is essential for ... | |
| 2000 | T V Rao, M T Haber, Jane M Sayer, Donald M Jerina | Incorporation of 4-thiothymidine into... | |
| 2000 | Leonid Dzantiev, Louis J Romano | A conformational change in E. coli DN... | |
| 2000 | Leonid Dzantiev, Louis J Romano | Differential effects of N-acetyl-2-am... | |
| 2000 | E F da Silva, Linda J Reha-Krantz | Dinucleotide repeat expansion catalyz... | |
| 1999 | Wai-Chung Lam, Edwin J C Van der Schans, L C Sowers, David P Millar | Interaction of DNA polymerase I (Klen... | |
| 1999 | C A Brautigam, K Aschheim, Thomas A Steitz | Structural elucidation of the binding... | Other Important Residues,All properties |
| 1999 | C A Brautigam, Siyang Sun, J A Piccirilli, Thomas A Steitz | Structures of normal single-stranded ... | |
| 1999 | Olga Rechkoblit, Shantu Amin, Nicholas E Geacintov | Primer length dependence of binding o... | |
| 1999 | Leonid Dzantiev, Louis J Romano | Interaction of Escherichia coli DNA p... | |
| 1999 | Dana T Minnick, Katarzyna Bebenek, Wendy P Osheroff, Robert M Turner, Mekbib Astatke, Lingfeng Liu, Thomas A Kunkel, Catherine M Joyce | Side chains that influence fidelity a... | |
| 1998 | Wai-Chung Lam, Edwin J C Van der Schans, Catherine M Joyce, David P Millar | Effects of mutations on the partition... | |
| 1998 | Mekbib Astatke, K Ng, Nigel D F Grindley, Catherine M Joyce | A single side chain prevents Escheric... | |
| 1998 | W S Furey, Catherine M Joyce, M A Osborne, D Klenerman, J A Peliska, S Balasubramanian | Use of fluorescence resonance energy ... | |
| 1998 | T E Carver, David P Millar | Recognition of sequence-directed DNA ... | |
| 1998 | A A Purmal, J P Bond, B A Lyons, Y W Kow, Susan S Wallace | Uracil glycol deoxynucleoside triphos... | |
| 1998 | Toshinori Suzuki, Masaru Yoshida, Masami Yamada, Hiroshi Ide, M Kobayashi, K Kanaori, K Tajima, K Makino | Misincorporation of 2'-deoxyoxanosine... | |
| 1998 | Yoshiyuki Mizushina, T Ueno, Y Goto, Y Isobe, M Sako, T Fujita, Kouji Hirota, H Hayashi, Kengo Sakaguchi | Carboxyflavins, novel inhibitors of T... | |
| 1998 | Olga I Lavrik, Heinz Peter Nasheuer, Klaus Weisshart, M S Wold, Rajendra Prasad, William A Beard, Samuel H Wilson, Alain Favre | Subunits of human replication protein... | |
| 1998 | Mekbib Astatke, Nigel D F Grindley, Catherine M Joyce | How E. coli DNA polymerase I (Klenow ... | |
| 1998 | M J Guo, S Hildbrand, C J Leumann, L W McLaughlin, M J Waring | Inhibition of DNA polymerase reaction... | |
| 1998 | S W Graves, Allison A Johnson, Kenneth A Johnson | Expression, purification, and initial... | Processivity,Kd,Vmax,All properties |
| 1998 | James R Kiefer, C Mao, J C Braman, Lorena S Beese | Visualizing DNA replication in a cata... | Specific Activity,Processivity,3-5' exo,KM,kcat,Polymerase Catalytic Residue Amino Acids,Residues Involved in Catalysis of 3-5' Exo,All properties |
| 1997 | E Bedford, Stanley Tabor, Charles C Richardson | The thioredoxin binding domain of bac... | Specific Activity,Processivity,All properties |
| 1997 | Thomas E Spratt | Klenow fragment-DNA interaction requi... | |
| 1997 | Marc M Greenberg, T J Matray | Inhibition of klenow fragment (exo-) ... | |
| 1997 | Holly Miller, Arthur P Grollman | Kinetics of DNA polymerase I (Klenow ... | Template lesions,All properties |
| 1996 | Dana T Minnick, Mekbib Astatke, Catherine M Joyce, Thomas A Kunkel | A thumb subdomain mutant of the large... | Cloned or native,Tagged,Full length or truncated,Frameshift Error Rate,Nucleotide Substitution Rate,KM,Kd,kcat,Molecular Weight,Overall Error Rate,All properties |
| 1996 | B M Moore, R K Jalluri, Michael B Doughty | DNA polymerase photoprobe 2-[(4-azido... | |
| 1996 | J S King, C F Fairley, W F Morgan | DNA end joining by the Klenow fragmen... | |
| 1996 | B M Moore, Ke Li, Michael B Doughty | Deoxyadenosine-based DNA polymerase p... | |
| 1996 | T I Kolocheva, A S Levina, G A Nevinsky | Recognition of the primers containing... | |
| 1996 | M W Frey, S T Frey, W D Horrocks, B F Kaboord, Stephen J Benkovic | Elucidation of the metal-binding prop... | |
| 1995 | Mekbib Astatke, Nigel D F Grindley, Catherine M Joyce | Deoxynucleoside triphosphate and pyro... | |
| 1995 | Hiroyuki Kamiya, Hironori Kasai | Formation of 2-hydroxydeoxyadenosine ... | KM,Vmax,All properties |
| 1995 | M W Frey, L C Sowers, David P Millar, Stephen J Benkovic | The nucleotide analog 2-aminopurine a... | |
| 1995 | Hiroyuki Kamiya, Tadamasa Ueda, T Ohgi, Akio Matsukage, Hironori Kasai | Misincorporation of dAMP opposite 2-h... | |
| 1995 | R Doisy, M S Tang | Effect of aminofluorene and (acetylam... | |
| 1995 | Catherine M Joyce, V Derbyshire | Purification of Escherichia coli DNA ... | |
| 1995 | V Derbyshire, J K Pinsonneault, Catherine M Joyce | Structure-function analysis of 3'-->5... | |
| 1995 | Katarzyna Bebenek, Thomas A Kunkel | Analyzing fidelity of DNA polymerases. | Frameshift Error Rate,Overall Error Rate,All properties |
| 1995 | Stephen J Benkovic, Craig E Cameron | Kinetic analysis of nucleotide incorp... | |
| 1995 | T J Matray, K J Haxton, Marc M Greenberg | The effects of the ring fragmentation... | |
| 1994 | T C Lin, G Karam, William H Konigsberg | Isolation, characterization, and kine... | KM,kcat,All properties |
| 1994 | T E Carver, R A Hochstrasser, David P Millar | Proofreading DNA: recognition of aber... | |
| 1994 | A A Purmal, Y W Kow, Susan S Wallace | 5-Hydroxypyrimidine deoxynucleoside t... | |
| 1994 | J G Hacia, P B Dervan, B J Wold | Inhibition of Klenow fragment DNA pol... | |
| 1994 | F J Wang, L S Ripley | DNA sequence effects on single base d... | |
| 1994 | D Simha, D Yadav, R W Rzepka, V A Palejwala, M Zafri Humayun | Base incorporation and extension at a... | |
| 1994 | P N Yadav, J S Yadav, Edward Arnold, Mukund J Modak | A computer-assisted analysis of conse... | |
| 1993 | Kristin A Eckert, Thomas A Kunkel | Fidelity of DNA synthesis catalyzed b... | |
| 1993 | A T Bankier | Dideoxy sequencing reactions using Kl... | |
| 1993 | Lorena S Beese, J M Friedman, Thomas A Steitz | Crystal structures of the Klenow frag... | Amino Acids Contacting NTP,All properties |
| 1993 | Lorena S Beese, V Derbyshire, Thomas A Steitz | Structure of DNA polymerase I Klenow ... | Amino Acids Contacting Template,Residues Involved in Catalysis of 3-5' Exo,Other Important Residues,All properties |
| 1993 | Thomas A Steitz, S Smerdon, J Jäger, Jianhong Wang, L A Kohlstaedt, J M Friedman, Lorena S Beese, P A Rice | Two DNA polymerases: HIV reverse tran... | |
| 1993 | H Kong, R B Kucera, William E Jack | Characterization of a DNA polymerase ... | Processivity,KM,All properties |
| 1993 | Linda B Bloom, M R Otto, J M Beechem, Myron F Goodman | Influence of 5'-nearest neighbors on ... | |
| 1993 | V Derbyshire, Mekbib Astatke, Catherine M Joyce | Re-engineering the polymerase domain ... | |
| 1993 | H P Rappaport | Replication of the base pair 6-thiogu... | |
| 1993 | W E Schy, L W Hertel, J S Kroin, Linda B Bloom, Myron F Goodman, Frank C Richardson | Effect of a template-located 2',2'-di... | |
| 1992 | P N Yadav, J S Yadav, Mukund J Modak | A molecular model of the complete thr... | |
| 1992 | M V Ljach, T I Kolocheva, V V Gorn, A S Levina, G A Nevinsky | The affinity of the Klenow fragment o... | |
| 1992 | Daekyu Sun, Laurence H Hurley | Effect of the (+)-CC-1065-(N3-adenine... | |
| 1992 | M Bakhanashvili, A Hizi | Fidelity of the reverse transcriptase... | RT,3-5' exo,All properties |
| 1992 | Catherine M Joyce, Xiaojun Chen Sun, Nigel D F Grindley | Reactions at the polymerase active si... | |
| 1992 | Linda J Reha-Krantz | Are there highly conserved DNA polyme... | |
| 1991 | M E Dahlberg, Stephen J Benkovic | Kinetic mechanism of DNA polymerase I... | |
| 1991 | G del Solar, M Espinosa | Labelling DNA ends with the Klenow fr... | |
| 1991 | Steven S Carroll, M Cowart, Stephen J Benkovic | A mutant of DNA polymerase I (Klenow ... | |
| 1991 | I A Lokhova, G A Nevinsky, T S Godovikova, E M Ivanova, A A Koshkin, D S Sergeev, E I Frolova, N K Rudenko, V V Khomov, V M Kavsan | 5'-derivatives of oligonucleotides as... | |
| 1991 | Lorena S Beese, Thomas A Steitz | Structural basis for the 3'-5' exonuc... | Residues Involved in Catalysis of 3-5' Exo,Other Important Residues,All properties |
| 1991 | B T Eger, Robert D Kuchta, Steven S Carroll, P A Benkovic, M E Dahlberg, Catherine M Joyce, Stephen J Benkovic | Mechanism of DNA replication fidelity... | |
| 1991 | C R Guest, R A Hochstrasser, C G Dupuy, D J Allen, Stephen J Benkovic, David P Millar | Interaction of DNA with the Klenow fr... | |
| 1991 | V Derbyshire, Nigel D F Grindley, Catherine M Joyce | The 3'-5' exonuclease of DNA polymera... | Cloned or native,Tagged,Full length or truncated,Specific Activity,3-5' exo,KM,kcat,All properties |
| 1991 | T K McDaniel, Y Huang, Jun Yin, S W Needleman, S J Meltzer | Direct radioactive labeling of unpuri... | |
| 1990 | J Rush, William H Konigsberg | Photoaffinity labeling of the Klenow ... | |
| 1990 | I A Potapova, G A Nevinsky, V V Khomov, Olga I Lavrik | NaF and mononucleotides as inhibitors... | |
| 1990 | A H Polesky, Thomas A Steitz, Nigel D F Grindley, Catherine M Joyce | Identification of residues critical f... | 3-5' exo,Molecular Weight,All properties |
| 1990 | M K Dosanjh, John M Essigmann, Myron F Goodman, B Singer | Comparative efficiency of forming m4T... | |
| 1990 | Katarzyna Bebenek, Catherine M Joyce, M P Fitzgerald, Thomas A Kunkel | The fidelity of DNA synthesis catalyz... | 3-5' exo,Frameshift Error Rate,Nucleotide Substitution Rate,Residues Involved in Catalysis of 3-5' Exo,All properties |
| 1990 | I A Potapova, G A Nevinsky, A G Veniaminova, V V Khomov, Olga I Lavrik | Interaction of dNTP, pyrophosphate an... | |
| 1990 | Vladimir N Podust, T O Korobeinicheva, G A Nevinsky, A S Levina, Olga I Lavrik | Inactivation of DNA polymerase by ade... | |
| 1990 | G A Nevinskiĭ, I A Potapova, N B Tarusova, O V Khalabuda, V V Khomov | [Interaction of dNTP-binding sites of... | |
| 1989 | S V Doronin, G A Nevinsky, T O Malygina, Vladimir N Podust, V V Khomov, Olga I Lavrik | The efficiency of interaction of deox... | |
| 1989 | D J Allen, P L Darke, Stephen J Benkovic | Fluorescent oligonucleotides and deox... | |
| 1989 | B Singer, F Chavez, S J Spengler, J T Kuśmierek, L Mendelman, Myron F Goodman | Comparison of polymerase insertion an... | |
| 1989 | C E Catalano, Stephen J Benkovic | Inactivation of DNA polymerase I (Kle... | |
| 1989 | G A Nevinsky, A V Nemudraya, A S Levina, V V Khomov | The algorithm of estimation of the Km... | |
| 1989 | D J Allen, Stephen J Benkovic | Resonance energy transfer measurement... | |
| 1989 | T A Rozovskaia, Sh Kh Minasian, M K Kukhanova, A A Kraevskiĭ, Z G Chidzhavadze | [DNA polymerase I, Klenow fragment of... | |
| 1989 | C Papanicolaou, L S Ripley | Polymerase-specific differences in th... | Overall Error Rate,All properties |
| 1989 | P Keohavong, William G Thilly | Fidelity of DNA polymerases in DNA am... | Nucleotide Substitution Rate,All properties |
| 1989 | Katarzyna Bebenek, Thomas A Kunkel | The use of native T7 DNA polymerase f... | |
| 1988 | H M Lim, J J Pène | Optimal conditions for supercoil DNA ... | |
| 1988 | V Derbyshire, P S Freemont, M R Sanderson, L Beese, J M Friedman, Catherine M Joyce, Thomas A Steitz | Genetic and crystallographic studies ... | Cloned or native,Tagged,Full length or truncated,Specific Activity,Residues Involved in Catalysis of 3-5' Exo,Other Important Residues,All properties |
| 1988 | P S Freemont, J M Friedman, Lorena S Beese, M R Sanderson, Thomas A Steitz | Cocrystal structure of an editing com... | Residues Involved in Catalysis of 3-5' Exo,Other Important Residues,All properties |
| 1988 | K R Tindall, Thomas A Kunkel | Fidelity of DNA synthesis by the Ther... | Full length or truncated,3-5' exo,Nucleotide Substitution Rate,Overall Error Rate,All properties |
| 1988 | R K Saiki, D H Gelfand, S Stoffel, S J Scharf, R Higuchi, G T Horn, K B Mullis, H A Erlich | Primer-directed enzymatic amplificati... | Nucleotide Substitution Rate,All properties |
| 1988 | P Keohavong, A G Kat, N F Cariello, William G Thilly | DNA amplification in vitro using T4 D... | |
| 1987 | Thomas A Steitz, L Beese, P S Freemont, J M Friedman, M R Sanderson | Structural studies of Klenow fragment... | |
| 1987 | M S Krug, S L Berger | First-strand cDNA synthesis primed wi... | |
| 1986 | P S Freemont, David L Ollis, Thomas A Steitz, Catherine M Joyce | A domain of the Klenow fragment of Es... | |
| 1986 | Thomas A Steitz, P S Freemont, David L Ollis, Catherine M Joyce, J M Grindley | Functional implications of the Klenow... | |
| 1985 | David L Ollis, C Kline, Thomas A Steitz | Domain of E. coli DNA polymerase I sh... | |
| 1985 | David L Ollis, P Brick, R Hamlin, N G Xuong, Thomas A Steitz | Structure of large fragment of Escher... | Cloned or native,Tagged,Full length or truncated,Other Important Residues,All properties |
| 1984 | A P Gupta, P A Benkovic, Stephen J Benkovic | The effect of the 3',5' thiophosphory... | |
| 1984 | V Bailly, W G Verly | The excision of AP sites by the 3'-5'... | |
| 1983 | Catherine M Joyce, Nigel D F Grindley | Construction of a plasmid that overpr... | Cloned or native,All properties |
| 1972 | D Brutlag, Arthur Kornberg | Enzymatic synthesis of deoxyribonucle... | Vmax,All properties |
| 1972 | P Setlow, D Brutlag, Arthur Kornberg | Deoxyribonucleic acid polymerase: two... | Specific Activity,Extinction Coefficient,3-5' exo,5-3' exo,Molecular Weight,All properties |
| 1972 | P Setlow, Arthur Kornberg | Deoxyribonucleic acid polymerase: two... | 3-5' exo,5-3' exo,Molecular Weight,All properties |
| 1970 | H Klenow, I Henningsen | Selective elimination of the exonucle... | Cloned or native,Tagged,Specific Activity,5-3' exo,Molecular Weight,All properties |
| Name | 3'-5' exo activity | 5'-3' exo activity | Frameshift Rate | Substitution Rate | General Error Rate |
|---|---|---|---|---|---|
| KF D355A | |||||
| KF L361A | |||||
| KF L361M | |||||
| KF D424E | |||||
| KF D424N | |||||
| KF D424A | No (2) | 5.0e-06-4.3e-03 errors/bp (2) | 2.5e-05-1.2e-04 errors/bp (2) | ||
| Klenow Fragment D424A | No (1) | No (1) | |||
| KF D705A | |||||
| KF S751C | |||||
| KF Y766S | |||||
| KF D882A | |||||
| KF C907S | |||||
| KF(515-928) | No (1) | 1.3e-05 errors/bp (1) | 3.2e-05 errors/bp (1) |
Digestion of: Eco Pol I with
Protein information: search for Klenow fragment at UniProt
Sequence information: